Greg Peterson starts for USA away to Chile
The match in Santiago kicks off at 11pm UK-time, with the return leg to be played in Glendale Colorado on Saturday July 16.
The winner over the two legs will secure a place in Rugby World Cup Pool D alongside England, Argentina, Japan and Samoa, while the loser gets one last shot at qualification later this year.
The USA warmed up for the game by beating the French Barbarians 26-21 last weekend in Houston – a game in which Peterson started.
The Eagles are coached by former Newcastle Falcons director of rugby Gary Gold, who said: “I am incredibly proud of the performance we put out in Houston.
“It was a short assembly period with the full squad, so I’m pleased with our early defensive effort and expect to see the same this Saturday.
“We allowed the French Barbarian’s back in the game as time went on last week with a few corrections needed at our set piece, but I’m confident we can play our game as we need to on the road.
“South America is always a challenging arena to play in on the road, so setting the pace early as we did last week, will be important against this hungry Chilean side.”